ENDOTHELIAL DYSFUNCTION IN CHRONIC OPISTORCHIASIS

УДК 616.12-009.86:611.018.74:616.995.122

Authors

  • Andrey P. Momot Altai State Medical University, Barnaul, Russia; Altai Branch of the National Medical Research Center for Hematology, Ministry of Health of Russia, Barnaul, Russia https://orcid.org/0000-0002-8413-5484 Email: xyzan@yandex.ru
  • Alexey V. Kudinov Altai State Medical University, Barnaul, Russia; Altai Branch of the National Medical Research Center for Hematology, Ministry of Health of Russia, Barnaul, Russia https://orcid.org/0000-0002-0967-6117 Email: kudinovalexej@gmail.com
  • Nina V. Karbysheva Altai State Medical University, Barnaul, Russia https://orcid.org/0000-0001-8320-3468 Email: nvk80@rambler.ru
  • Olga V. Beskhlebova Altai State Medical University, Barnaul, Russia https://orcid.org/0000-0003-4561-1019 Email: olg.deriglazova@yandex.ru
  • Marina A. Nikonorova Altai State Medical University, Barnaul, Russia https://orcid.org/0000-0001-6621-9310 Email: ma.nikulina@mail.ru
  • Maria E. Ivanova Altai State Medical University, Barnaul, Russia Email: ivanova.akcomd@yandex.ru
  • Dina A. Trukhina Altai State Medical University, Barnaul, Russia Email: dina.truhina@yandex.ru

DOI:

https://doi.org/10.31684/25418475-2023-4-16

Keywords:

chronic opisthorchiasis, endothelial dysfunction, extracellular vesicles, flow cytometry

Abstract

Relevance. The basis of the complex, in some cases, of interrelated pathological processes in opisthorchiasis invasion may be endothelial dysfunction, which provokes the risks of cardiovascular events, and is an important aspect in establishing pathogenetic connections between diseases of the liver and the cardiovascular system. When assessing the inflammatory process in the pathogenesis of invasion, there is increasing interest in the contribution of not only cellular elements and circulating inflammatory markers, but also in intercellular communication and regulation through microvesicles. Purpose of the study. To study the circulation of extracellular vesicles, which are associated with blood and endothelial cells, in patients with chronic opisthorchiasis. Materials and methods. The study was conducted on two groups of people (men and women aged 18 to 55 years (34.3±1.3 years)). The experimental group included patients with chronic opisthorchiasis (n=26). There was the control group (n=14) matched by gender and age with experimental group. The qualitative composition and quantity of extracellular vesicles (EVs) was determined by flow cytometry. Results. In the experimental group, there was a significant increase in the number of CD41+ events (platelet EVs) 2 times (from 120 to 245 events/μl (p = 0.030), CD45+ events (panleukocyte EVs) - more than 120 times (from 95 to 11713 events/μl (p=0. 0330), as well as a trend towards an increase in the rate for CD31+ events by 19.8 times (from 495 to 9818 events/μl (p=0.133) in comparison with control group. Screening assessment of the CD9+ population of platelet EVs (CD41+31+CD9+), endothelial EVs (CD41-31+9+) and leukocyte EVs (CD45+CD9+) showed a significant (p=0.0330) increase in events, while their content corresponded to the data obtained by CD31+41+ events in quantitative terms. For endothelial EVs, there was a significant increase in the proportion of CD9+ events from 23.3% in the control to 46.2% in the experimental group, while for leukocyte EVs there was no statistically significant change in the percentage ratio, and it was 9.9% in the control and 12.9% in the experimental group. Conclusions. These results can be considered from the perspective of platelet activation, as well as the development of the inflammatory process and endothelial dysfunction, where microvesicles can act as potential highly sensitive markers for opisthorchiasis invasion.

Downloads

Download data is not yet available.

Author information

Andrey P. Momot,
Altai State Medical University, Barnaul, Russia; Altai Branch of the National Medical Research Center for Hematology, Ministry of Health of Russia, Barnaul, Russia

Dr. Sci. (Med.), Professor, Head of the Hemostasis Laboratory, Institute of Clinical Medicine, Altai State Medical University; Director, Altai Branch of National Medical Research Center for Hematology, Barnaul.
656045, Barnaul, Lyapidevskogo Str., 1.
E-mail: xyzan@yandex.ru
https://orcid.org/0000-0002-8413-5484

Alexey V. Kudinov,
Altai State Medical University, Barnaul, Russia; Altai Branch of the National Medical Research Center for Hematology, Ministry of Health of Russia, Barnaul, Russia

Cand. Sci. (Biol.), Senior Researcher, Hemostasis Laboratory, Institute of Clinical Medicine, Altai State Medical University; Researcher, Altai Branch of National Medical Research Center for Hematology, Barnaul.
E-mail: kudinovalexej@gmail.com.
https://orcid.org/0000-0002-0967-6117

Nina V. Karbysheva,
Altai State Medical University, Barnaul, Russia

Dr. Sci. (Med.), Professor, Head of the Department of Infectious Diseases with Advanced Training Course, Altai State Medical University, Barnaul.
656038, Altai Krai, Barnaul, Lenina Ave., 40.
E-mail: nvk80@rambler.ru. Tel: +7 (3852) 566-964.
https://orcid.org/0000-0001-8320-3468

Olga V. Beskhlebova,
Altai State Medical University, Barnaul, Russia

Cand. Sci. (Med.), Associate Professor, Department of Infectious Diseases with Advanced Training Course, Altai State Medical University, Barnaul. E-mail: olg.deriglazova@yandex.ru.
https://orcid.org/0000-0003-4561-1019

Marina A. Nikonorova,
Altai State Medical University, Barnaul, Russia

Dr. Sci. (Med.), Associate Professor, Professor of the Department of Infectious Diseases with Advanced Training, Altai State Medical University, Barnaul.
E-mail: ma.nikulina@mail.ru.
https://orcid.org/0000-0001-6621-9310

Maria E. Ivanova,
Altai State Medical University, Barnaul, Russia

Assistant of the Department of Infectious Diseases with Advanced Training Course, Altai State Medical University, Barnaul.
E-mail: ivanova.akcomd@yandex.ru

Dina A. Trukhina,
Altai State Medical University, Barnaul, Russia

Junior Researcher, Laboratory of Epidemiology and Evidence-based Medicine, Altai State Medical University, Barnaul.
E-mail: dina.truhina@yandex.ru

References

  • Grebenchikov O.A., Dolgikh V.T., Prokofiev M.D. Endothelial Dysfunction as the most important pathogenetic factor in the development of a critical condition. Bulletin of SurGU. Medicine. 2021; 3(49): 51-60. (In Russ.)
  • Ignatiev S.V., Lyanguzov A.V. The functional role of microvesicles in normal and pathological conditions. Clinical physiology of blood circulation. 2019; 16(4): 261-266. (In Russ.)
  • Rackov G., Garcia-Romero N., Esteban-Rubio S., Carrión-Navarro J. et al. Vesicle-mediated control of cell function: the role of extracellular matrix and microenvironment. Frontiers in Physiology. 2018; 9(651): 1-12. https://doi.org/10.3389/fphys.2018.00651
  • Novikova M.A., Savich Yu.V., Lasyukov E.A., Frolova R.L. Extracellular vesicles: biological properties and their therapeutic potential (review of current information). Laboratory diagnostics. Eastern Europe. 2021; 10(4): 412-419. (In Russ.)
  • Ohayon L., Zhang X., Dutta P. The role of extracellular vesicles in regulating local and systemic inflammation in cardiovascular disease. Pharmacol Res. 2021; 170(105692): 1-12. https://doi.org/10.1016/j.phrs.2021.105692.
  • Peng M., Liu X., Xu G.J. Extracellular Vesicles as Messengers in Atherosclerosis. Cardiovasc Transl Res. 2020; 13(2): 121-130. https://doi.org/10.1007/s12265-019-09923- z.
  • Brown P.A., Brown P.D. Extracellular vesicles and atherosclerotic peripheral arterial disease. Cardiovasc. Pathol. 2023; 63: 107510. https://doi.org/10.1016/j.carpath.2022.107510
  • Yang J., Zou X., Jose P.A., Zeng C. Extracellular vesicles: Potential impact on cardiovascular diseases. Adv Clin Chem. 2021; 105: 49-100. https://doi.org/10.1016/bs.acc.2021.02.002
  • Garcia-Romero N., Esteban-Rubio S., Rackov G., Carrión-Navarro J. et al. Extracellular vesicles compartment in liquid biopsies: clinical application. Molecular Aspects of Medicine. 2018; 60: 27-37. https://doi.org/10.1016/j.mam.2017.11.009
  • Schindler S.M., Little J.P., Klegeris A. Microparticles: a new perspective in central nervous system disorders. BioMed Research International. 2014; 2014 (756327): 1-17. https://doi.org/10.1155/2014/756327
  • Sirotkina O.V., Ermakov A.I., Zhilenkova Yu.I., Zolotova E.A., Kudlay D.A., Vavilova T.V., Gaykova L.B. Dynamics of formation of microvesicles of blood cells in COVID-19 patients at different stages of the disease. Preventive and clinical medicine. 2021; 4(81): 68-74. (In Russ.)
  • Lugo-Gavidia L.M., Burger D., Matthews V.B., Nolde J.M. et al. Role of Microparticles in Cardiovascular Disease: Implications for Endothelial Dysfunction, Thrombosis, and Inflammation. Hypertension. 2021; 77(6): 1825-1844. https://doi.org/10.1161/HYPERTENSIONAHA.121.16975
  • Karbysheva N.V. Complex approach to the diagnosis of chronic opisthorchiasis. Bulletin of medical science. 2017; 1(5): 75-78. https://doi.org/10.31684/2541-8475.2017.1(5).75-78. EDN ZQJALX.
    • Том 32 № 4 (2023): Бюллетень медицинской науки

    Downloads

    Published

    2023-12-22

    How to Cite

    Momot A. P., Kudinov A. V., Karbysheva N. V., Beskhlebova O. V., Nikonorova M. A., Ivanova M. E., Trukhina D. A. ENDOTHELIAL DYSFUNCTION IN CHRONIC OPISTORCHIASIS: УДК 616.12-009.86:611.018.74:616.995.122 // Bulletin of Medical Science, 2023. Vol. 32, № 4. P. 16–24 DOI: 10.31684/25418475-2023-4-16. URL: https://newbmn.asmu.ru/bmn/article/view/628.

    Issue

    Vol. 32 No. 4 (2023): Bulletin of Medical Science

    Section

    ORIGINAL PAPERS

    License

    Creative Commons License

    This work is licensed under a Creative Commons Attribution 4.0 International License.

    Most read articles by the same author(s)