LACTOFERRIN AS A DIAGNOSTIC MARKER FOR CONGENITAL INFECTIOUS DISEASES IN NEWBORNS OF MOTHERS WITH ACUTE CHLAMYDIA INFECTION

UDC 618.3-06:616.98-053.3

Authors

  • Veronika S. Grebneva Novokuznetsk State Institute for Advanced Training of Physicians, a branch of the Russian Medical Academy of Continuing Professional Education, Novokuznetsk Email: veronika071988@yandex.ru
  • Lyudmila V. Renge Novokuznetsk State Institute for Advanced Training of Physicians, a branch of the Russian Medical Academy of Continuing Professional Education, Novokuznetsk https://orcid.org/0000-0002-7237-9721 Email: renge@mail.ru
  • Ekaterina Yu. Grigorieva Novokuznetsk State Institute for Advanced Training of Physicians, a branch of the Russian Medical Academy of Continuing Professional Education, Novokuznetsk Email: prutovykh@icloud.com
  • Anna E. Vlasenko Novokuznetsk State Institute for Advanced Training of Physicians, a branch of the Russian Medical Academy of Continuing Professional Education, Novokuznetsk https://orcid.org/0000-0001-6454-4216 Email: vlasenkoanna@inbox.ru

DOI:

https://doi.org/10.31684/25418475-2023-2-24

Keywords:

congenital infection, Chlamydia trachomatis, lactoferrin

Abstract

The purpose of the study: The possibility of using the determination of the level of lactoferrin (LF) in the blood and amniotic fluid of pregnant women with acute chlamydia infection in the diagnosis of congenital infectious diseases of the fetus and newborn. Materials and methods. Venous blood and amniotic fluid were studied in 125 women at 37-41 weeks of gestation with chlamydia infection with various variants of its course. The first group included 83 pregnant women with acute chlamydia infection (PCR test +, IdM+, IgG+/-); of which 44 had children with congenital infectious diseases and 39 had healthy children. The second group (comparison group) consisted of 42 pregnant women with Chlamydia trachomatis (PCR test, IdM, IgG+), of which 22 had newborns with VIZ and 20 had healthy children. Comparing the indicators analyzed with the median measured in women without infectious load who gave birth to healthy children, these estimates were obtained from the database No. 2020621918 "Clinical characteristics of pregnant women taking into account the presence and severity of intrauterine infection". The level of lactoferrin in the blood serum and amniotic fluid was determined by solid-phase enzyme immunoassay. Checking the normality of the distribution (the Shapiro-Wilkes criterion was applied) revealed statistically significant deviations from the normal distribution law for all features considered. The descriptive statistics of the quantitative characteristics are represented by the median and interquartile range – Med(Q1-Q3). The comparison of 4 groups among themselves on a quantitative basis was carried out using the Kraskel-Wallis criterion, and a posteriori comparisons were carried out using the Dunn criterion. Comparison of the indicator in each group with the median measured in healthy women was performed using a single-sample Wilcoxon criterion. Results. In the blood serum of pregnant women with acute infection, as well as carriers of Chlamydia trachomatis and the birth of VIZ-positive children, an increased concentration of lactoferrin (LF <4.0 g /l-;) was found, compared to protein indicators in pregnant women who gave birth to healthy children. The level of LF in the amniotic fluid was statistically significantly lower in the acute form of chlamydia infection and in the birth of children with congenital infectious diseases. Conclusion. Determinement of lactoferrin levels in blood serum and amniotic fluid in pregnant women with CHI allowed us to identify a prenatal diagnostic criterion for congenital infectious diseases of the fetus and newborn.

Downloads

Download data is not yet available.

Author information

Veronika S. Grebneva,
Novokuznetsk State Institute for Advanced Training of Physicians, a branch of the Russian Medical Academy of Continuing Professional Education, Novokuznetsk

Assistant of the Department of Obstetrics and Gynecology, Novokuznetsk State Institute for Advanced Training of Physicians, Branch "Russian Medical Academy of Continuing Professional Education", Novokuznetsk.
654005, Russia, Novokuznetsk, Stroiteley Ave. 5.
E-mail: veronika071988@yandex.ru, Tel.: +7(3843)454-873
https://orсid.org/0000-0003-4546-0046

Lyudmila V. Renge,
Novokuznetsk State Institute for Advanced Training of Physicians, a branch of the Russian Medical Academy of Continuing Professional Education, Novokuznetsk

Dr. Sci. (Med.), Associate Professor, Head of the Department of Obstetrics and Gynecology, Novokuznetsk State Institute for Advanced Medical Education, Branch of "Russian Medical Academy of Continuing Professional Education", Novokuznetsk.
654005, Russia, Novokuznetsk, Stroiteley pr. 5.
E-mail: renge@mail.ru, Tel.: +7(3843)454-873
https://orcid.org/0000-0002-7237-9721

Ekaterina Yu. Grigorieva,
Novokuznetsk State Institute for Advanced Training of Physicians, a branch of the Russian Medical Academy of Continuing Professional Education, Novokuznetsk

Cand. Sci. (Med.), Associate Professor, Department of Obstetrics and Gynecology, Novokuznetsk State Institute for Advanced Medical Training, Novokuznetsk Branch of "Russian Medical Academy of Continuing Professional Education", Novokuznetsk.
654005, Russia, Novokuznetsk, Stroiteley Ave. 5.
E-mail: prutovykh@icloud.com, Tel.: +7(3843)454-873
https://orcid.org/0000-0002-8623-729Х

Anna E. Vlasenko,
Novokuznetsk State Institute for Advanced Training of Physicians, a branch of the Russian Medical Academy of Continuing Professional Education, Novokuznetsk

Cand. Sci. (Tech.), Lecturer, Department of Medical Cybernetics and Informatics, Novokuznetsk State Institute for Advanced Medical Training, Branch "Russian Medical Academy of Continuing Professional Education", Novokuznetsk.
654005, Russia, Novokuznetsk, Stroiteley Ave. 5
E-mail: vlasenkoanna@inbox.ru, Tel.: +7(3843)454-873
https://orcid.org/0000-0001-6454-4216

References

  • Tirskaya Yu. I., Belyakova T. N., Shamina I. V., Barabchik I. A., Chulovsky Yu. I., Korneev B. V. Immunological features of pregnant women at risk for intrauterine infection. Journal of Siberian Medical Sciences. 2014; 2: 16. (In Russian)]. URL: https://mos.ngmu.ru/article/text_full.php?id=1289
  • Shkarin V.V., Poslova L.Yu., Kovalishena O.V., Sergeeva A.V., Senyagina N.E. Epidemiology of intrauterine infections in newborns. Nizhnii Novgorod: Remedium Privolzh'e; 2019. 124 р. (In Russian). URL: https://www.elibrary.ru/download/elibrary_41568395_25281655.pdf
  • Novikova O.N., Ushakova G.A. Current approaches to the diagnosis of intrauterine infections. Russian Bulletin of Obstetrician-Gynecologist. 2016; 4: 36-43. (In Russian). https://doi.org/10.17116/rosakush201616436-43
  • Zheptisbaev F.A., Pushkareva K.A., Gulamova D.A., Ismailova G.A., Razieva R.Kh., Yakusheva A.O., et al. Chlamydial infection in newborns. Vestnik KazNMU. 2014; 1: 113-116. (In Russian).
  • Kisina V.I., Andreyeva I.V., Stetsyuk O.U. Treatment for chlamydia infection in gynecology and obstetrics: a review of current foreign and russian guidelines. Obstetrics and gynecology. 2012; 4/1: 91-98. (In Russian).
  • Tirskaya Yu.I., Belkova T.N. Calculation of the clinical and economic efficiency of the modified algorithm of examination of pregnant women for intrauterine infections. Klinicheskaya Laboratornaya Diagnostika (Russian Clinical Laboratory Diagnostics). 2012; 9: 9. (In Russian).
  • Borovik T.E., Yatsyk G.V., Namazova-Baranova L.S., Zvonkova N.G., Semenova N.N., Lukoyanova О.L., et al. Applicability of a human lactoferrin in pediatric practice. Current Pediatrics. 2014; 13(4): 12-19. (In Russian). https://doi.org/10.15690/vsp.v13i4.1079
  • Zorina V.N., Zorin N.A. Protein components of innate immunity in protection from pathogenic invasion (literature review). Zhurnal mikrobiologii, èpidemiologii i immunobiologii. 2013; 3: 111-117. (In Russian).
  • Zorina V.N. Pattern of lactoferrin anti-influenza virus inhibitory activity. Russian Journal of Infection and Immunity. 2020; 1: 49-54. (In Russian)]. https://doi.org/10.15789/2220-7619-POL-1156.
  • Małaczewska J., Kaczorek-Łukowska E., Wójcik R., Siwicki A. Antiviral effects of nisin, lysozyme, lactoferrin and their mixtures against bovine viral diarrhoea virus. BMC veterinary research. 2019; 15(1): 318-330. https://doi.org/10.1186/s12917-019-2067-6.
  • Zhilyakova E.T., Novikov O.O., Khmyrov A.V., Fadeeva D.A., Gulyaeva V.E., Malyutina A.Yu., Avtina N.V. Properties and Prospects of Application of the Whey Protein Lactoferrin in Medicine and Veterinary Medicine (Review). Drug development & registration. 2022; 11(1): 32-39. (In Russian). https://doi.org/10.33380/2305-2066-2022-11-1-32-39
  • Berlutti F., Pantanella F., Natalizi T., Frioni A., Paesano R., Polimeni A., Valenti P. Antiviral properties of lactoferrin − a natural immunity molecule. Molecules. 2011; 16(8): 6992-7018. https://doi.org/10.3390/molecules16086992
  • Legrand D., Elass E., Carpentier M., Mazurier J. Lactoferrin: a modulator of immune and inflammatory responses. Cell Mol Life Sci. 2005; 62(22): 2549-2559. https://doi.org/10.1007/s00018-005-5370-2
  • Puddu P., Latorre D., Carollo M., Catizone A., Ricci G., Valenti P., Gessani S. Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells. PLoS One. 2011; 6(7): e22504. https://doi.org/10.1371/journal.pone.0022504
  • Renge L.V., Bazhenova L.G., Zorina V.N., Zorina R.M., Chirikova T.S. Amniotic fluid immunoregulatory proteins in the mono- and mixed carriage of perinatal infection pathogens. Russian Bulletin of Obstetrician-Gynecologist. 2015; 15(6): 17-23. (In Russian). https://doi.org/10.17116/rosakush201515617-23
  • Iksanova A.M., Arzumanian V.G., Konanykhina S.Y., Samoylikov P.V. Antimicrobial peptides and proteins in human biological fluids. MIR J. 2022; 9(1): 37-55. (In Russian). https://doi.org/10.18527/2500-2236-2022-9-1-37-55/
  • Valenti P., Rosa L., Capobianco D. Lepanto M. S., Schiavi E., Cutone A. et al. Role of lactobacilli and lactoferrin in the mucosal cervicovaginal defense. Frontiers in immunology. 2018; 9: 376. https://doi.org/10.3389/fimmu.2018.00376
  • Kuznetsov I.A., Potiyevskaya V.I., Kachanov I.V., Kuraleva O.O. Laktoferrin''s role in biological circles of the person. Modern Problems of Science and Education. 2017; 3: 69. (In Russian).
  • Tsarev V.N., Goldman I.L., Sadchikova E.R., Ippolitov E.V., Podporin М.S. Assessing the impact of recombinant human lactoferrin on the characteristicsof the growth curves of bacterial pathogens populations. Natsional''nye prioritety rossii. 2016; 2: 130-133. (In Russian).
  • Aleshina G.M. Lactoferrin − an endogenous regulator of the protective functions of the organism. Medical academic journal. 2019; 19(1): 35-44. (In Russian). https://doi.org/10.17816/MAJ19135-44
  • Telang S. Lactoferrin: a critical player in neonatal host defense. Nutrients. 2018; 10(9): 1228. https://doi.org/10.3390/nu10091228.
  • Manzoni P., Dall’Agnola A., Tomé D., Kaufman D. A., Tavella E., Pieretto M. et al. Role of lactoferrin in neonates and infants: an update. American journal of perinatology. 2018; 35(6): 561-565. https://doi.org/10.1089/bfm.2019.0033.
  • Nikolaev A.A., Sukharev A.E. The structure and functions of lactoferrin and its role in human reproduction (a review). Russian Journal of Human Reproduction. 2015; 21(6): 33-38. (In Russian) https://doi.org/10.17116/repro201521625-30
  • Grigoryeva E.Yu., Renge L.V., Zorina V.N., Bazhenova L.G., Likhacheva V.V. Immunoregulatory and transport proteins as markers of antenatal prognosis of preterm infants' status in women with preterm prelabor rupture of membranes. Obstetrics and gynecology. 2020; 3: 148-154. (In Russian). https://dx.doi.org/10.18565/aig.2020.3.148-154
  • Renge L.V., Zorina V.N., Grebneva V.S., Grigoryeva E.Yu., Vlasenko A.E. Markers of permeability and functional state of the placental barrier in pregnant women with chlamydial infection. Obstetrics and gynecology. 2022; 6: 59-66. (In Russian). https://dx.doi.org/10.18565/aig.2022.6.59-66
  • Grebneva V.S., Renge L.V., Zorina V.N., Grigoryeva E.Yu., Vlasenko A.E. Chlamydia infection in pregnant women: possibilities of fetal and neonatal assessment using some acute-phase blood parameters and logistic regression methods. Mother and baby in Kuzbass. 2022; 3: 54-61. (In Russian). https://dx.doi.org/10.24412/2686-7338-2022-3-54-61
  • Certificate of state registration of the database No. 2020622019 Russian Federation. Clinical characteristics of pregnant women with premature rupture of the membranes, taking into account the presence and severity of intrauterine infection in a child at 24-33 weeks of gestation: № 2020621918 : appl. 2020.20.10 : publ. 23.10.2020 / A.E. Vlasenko, E.Yu. Grigoryeva, L.V. Renge. (In Russian).

    • Downloads

    Published

    2023-06-26

    How to Cite

    Grebneva V. S., Renge L. V., Grigorieva E. Y., Vlasenko A. E. LACTOFERRIN AS A DIAGNOSTIC MARKER FOR CONGENITAL INFECTIOUS DISEASES IN NEWBORNS OF MOTHERS WITH ACUTE CHLAMYDIA INFECTION: UDC 618.3-06:616.98-053.3 // Bulletin of Medical Science, 2023. Vol. 30, № 2. P. 24–34 DOI: 10.31684/25418475-2023-2-24. URL: https://newbmn.asmu.ru/bmn/article/view/567.

    Issue

    Vol. 30 No. 2 (2023): Bulletin of Medical Science

    Section

    ORIGINAL PAPERS

    License

    Copyright (c) 2023 Вероника Сергеевна Гребнева, Людмила Владимировна Ренге, Екатерина Юрьевна Григорьева, Анна Егоровна Власенко

    Creative Commons License

    This work is licensed under a Creative Commons Attribution 4.0 International License.

    Most read articles by the same author(s)